Laboratory Hematology Practice

Laboratory Hematology Practice


Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international
textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory
technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how
technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for
all those working in the field.





I Cellular Analysis, 1



1 Historical Perspective on Cellular Analysis, 3
Elkin Simson



2 Cellular Morphologic Analysis of Peripheral Blood, 10
Powers Peterson, Sheila McNeill, and Gene Gulati



3 Automated Cell Analysis: Principles, 26
Bruce H. Davis and Patrick W. Barnes



4 Differential Leukocyte Analysis, 33
Marie-Christine B??and Francis Lacombe



5 Automated Platelet Analysis, 48
Carol Briggs and Samuel J. Machin



6 Method Standardization in Cellular Analysis, 59
Ian Giles & Richard Kendall



7 Spurious counts and spurious results on hematology analyzers: platelets, 66
Marc Zandecki, Franck Genevieve, J??ie G?ard, and Alban Godon



8 Spurious Counts and Spurious Results on Hematology Analyzers: White Blood Cells, Red Blood Cells, Hemoglobin, Red
Cell Indices, and Reticulocytes, 79
Marc Zandecki, Franck Genevieve, J??ie G?ard, and Alban Godon



9 Evaluation of Hematology Analyzers, 96
Carol Briggs



II Flow Cytometry in Hematologic Diagnosis, 103



10 Flow Cytometric Specimen Collection, Processing, and Reporting, 105
Maryalice Stetler-Stevenson, Bruce Greig, Constance Yuan



11 Validation and Quality Control in Clinical Flow Cytometry, 115
Norman B. Purvis and Teri Oldaker



12 A New Paradigm for Cytometric Analysis, 131
C. Bruce Bagwell



III Molecular Diagnostics, 143



13 Sample Collection, Processing, and Storage for Molecular Genetic Testing, 145
Bobby L. Boyanton Jr. and Domnita Crisan



14 Development of Amplification-Based Molecular Genetic Testing in Hematology, 155
Raymond R. Tubbs



15 Single Nucleotide Polymorphisms in Molecular Diagnostics, 168
Gurunathan Murugesan, Seongsoo Jang, and Jin-Yeong Han



16 Molecular Cytogenetic (FISH) Analysis of Hematolymphoid Disorders, 181
Mark A. Micale and Anwar N. Mohamed



17 Immunoglobulin and T-Cell Receptor Gene Rearrangement Analysis for Diagnosis of Hematologic Malignancies, 199
Jacques J. M. van Dongen, Anton W. Langerak, and Tomasz Szczepan ski



18 Minimal Residual Disease Detection in Acute Myeloid Leukemia by RQ-PCR and Multiparameter Flow Cytometry, 220
Wolfgang Kern and Susanne Schnittger



19 Engraftment Analysis using Short Tandem Repeats following Allogeneic Hematopoietic Cell Transplantation, 234
Ilka Warshawsky and Hyun-Sook Chi



20 Molecular Profiling Methods in the Diagnosis of Hematologic Disorders, 244
Annette S. Kim, Stephen R. Master, and Cherie H. Dunphy



21 The Role of Classical Cytogenetics in Hematologic Diagnosis, 267
Kathleen Richkind



IV Bone Marrow Analysis, 279



22 Bone Marrow Processing and Normal Morphology, 281
Karl S. Theil



23 Cytochemical, Immunocytochemical, Histochemical, and Immunohistochemical Staining of Bone Marrow and Peripheral
Blood, 300
Alexandra Rice and Barbara J. Bain



24 The Myeloproliferative Neoplasms, 315
Sindhu Cherian and Brent L. Wood



25 Myelodysplastic Disorders, 331
Maryalice Stetler-Stevenson, Joseph A. DiGiuseppe, and Diane C. Arthur



26 Acute Leukemias, 345
Joseph A. DiGiuseppe and Laila Mnayer



27 Lymphoproliferative Disorders, 364
Robert W. Allan, Samer Z. Al-Quran, Ying Li, and Raul C. Braylan



28 Plasma Cell Disorders, 381
Bill G. Richendollar and James R. Cook



29 Diagnosis of Adult Mastocytosis: Role for Bone Marrow Analysis, 388
Luis Escribano, Andr? Garcia-Montero, Laura Sanchez-Mu?z, Cristina Teodosio, Ivan Alvarez-Twose, Maria Jara-Acevedo,
Jos?Mario Morgado, Julia Almeida, Alberto Orfao for the Spanish Network on Mastocytosis (REMA)



30 Paroxysmal Nocturnal Hemoglobinuria (PNH) Analysis, 399
Stephen J. Richards



V Hemostasis and Thrombosis, 407



31 Sample Collection and Processing in Hemostasis, 409
Dorothy M. Adcock



32 Performance and Interpretation of Routine Coagulation Assays, 420
Kandice Kottke-Marchant



33 Clotting Factors and Inhibitors: Assays and Interpretation, 435
Piet Meijer, H. W. Verbruggen, and Michael Spannagl



34 Diagnosis of von Willebrand Disease, 447
Emmanuel J. Favaloro and Jerry Koutts



35 Diagnosis of Fibrinolytic Disorders, 460
Wayne Chandler



36 Laboratory Evaluation of Acquired Coagulation Disorders, 468
Richard A. Marlar and Katerine Seywerd



37 Platelet Assays and Platelet Dysfunction, 480
Paul Harrison and David Keeling



38 Thrombophilia: Assays and Interpretation, 492
Sophia Yohe and John Olson



39 Lupus Anticoagulants, Antiphospholipid Antibodies, and Antiphospholipid Syndrome, 509
William L. Nichols, Kandice Kottke-Marchant, Marlies R. Ledford-Kraemer, Henry A. Homburger, Layna K. Cardel



40 Heparin-Induced Thrombocytopenia, 526
Sixten Selleng and Andreas Greinacher



41 Anticoagulant, Antiplatelet, and Thrombolytic Drugs, 535
Laura Green and Samuel J. Machin



42 Guidelines for Evaluation of Coagulation Analyzers and Coagulation Testing, 543
Chris Gardiner



VI Special Hematology Techniques, 553



43 Essentials of Immunohematology, 555
A. Bradley Eisenbrey



44 Analysis of Hemoglobinopathies, Hemoglobin Variants and Thalassemias, 562
Monica V. E. Gallivan and Piero C. Giordano



45 Nutritional Anemia, 586
Ralph Green



46 Hemolytic Anemias Associated with Disorders of Erythrocyte Membrane and Cytoskeleton Proteins, 602
May-Jean King, Lesley J. Bruce, Didier Dhermy



47 Body Fluid Analysis, 615
David J. Blomberg, Katherine Galagan, and Eric F. Glassy



48 Malaria Analysis, 626
Qigui Li, Peter J. Weina, and R. Scott Miller



49 Erythrocyte Sedimentation Rate (ESR), 638
Josep M. Jou



VII Point-of-Care Testing, 647



50 Needs Analysis and Selection of Point-of-Care Testing Analyzers, 649
Albert Huisman



51 Point-of-Care Testing in Hemostasis, 655
Steve Kitchen and Dianne Kitchen



52 Point-of-Care Hematology Assays, 668
Albert Huisman



VIII Management of Information 673



53 Laboratory Management, 675
Stefanie L. McFadden and Linda M. Sandhaus



54 Hematology Quality Practices 686
George S. Cembrowski



55 Digital Imaging in Hematology, 707
Danny Hsu and Szu-Hee Lee



Index, 719