In Vitro Mutagenesis Protocols-3판(Hardcover)

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions
of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox
containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence
function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully
employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of
biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a
guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful
Methods in Molecular Biology?series format, chapters include brief introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on
troubleshooting and avoiding known pitfalls.


Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable
compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological
implications.

Content Level ?Professional/practitioner

Keywords ?Microbial systems - Nucleotide sequences - PCR - Protein regulation - Protein structure-function - Protein-
protein interaction - Sequence function

Related subjects ?Biochemistry & Biophysics - Human Genetics