Condensed Protocols from Molecular Cloning : A Laboratory Manual

Trends in Cell Biology
This book should be the first port of call when establishing a molecular technique new to a
laboratory

Scientist
Any basic research laboratory using molecular biology techniques will benefit from having a copy on
hand..


--------------------------------------------------------------------------------



목차

차 례
1. Using Plasmid Vectors in Molecular Cloning
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
Appendices
Index