Microtubule Protocols(Hardcover)

This book contains a comprehensive collection of essential up-to-date
methods used to study the biology of microtubules and the mechanisms
of action of microtubule-interacting drugs. These convenient and
reproducible methods range from the purification and characterization
of microtubule proteins, analysis of post-translational modifications
of tubulin, and determination of microtubule structure, to
visualization of microtubule-mediated cellular process. This book
also includes techniques to examine drug-microtubule/tubulin
interactions and methods for the development and discovery of novel
agents that target microtubules.

Microtubule Protocols
Editor: Zhou, Jun (Nankai University, Tianjin, China)

Table of Contents:

Chapter 1 (review)
Microtubules: an overview
Richard H. Wade

Chapter 2
Large scale purification of brain tubulin with the modified
Weisenberg procedure
Jos?Manuel Andreu

Chapter 3
Purification and mass-spectrometry identification of microtubule-
binding proteins from Xenopus egg extracts
Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and
Andrei V. Popov

Chapter 4
Preparation and characterization of post-translationally modified
tubulins from Artemia Franciscana
Paul A. O'Connell and Thomas H. MacRae

Chapter 5
Studying the structure of microtubules by electron microscopy
Linda A. Amos and Keiko Hirose

Chapter 6
Fluorescence microscopy of microtubules in cultured cells
Irina Semenova and Vladimir Rodionov

Chapter 7 (review)
Measurements of stathmin-tubulin interaction in solution
Marie-France Carlier

Chapter 8
Analysis of microtubule dynamics by polarized light
Rudolf Oldenbourg

Chapter 9
Visualization of spindle behavior using confocal microscopy
Adam I. Marcus

Chapter 10
Live cell approaches for studying kinetochore-microtubule
interactions in Drosophila
Daniel W. Buster and David J. Sharp

Chapter 11
Analysis of tubulin transport in nerve processes
Andrey Tsvetkov and Sergey Popov

Chapter 12
Analysis of microtubule-mediated intracellular viral transport
Chunyong Liu, Min Liu and Jun Zhou

Chapter 13
Xenopus oocyte wound healing as a model system for analysis of
microtubule-actin interactions
Tong Zhang and Craig A. Mandato

Chapter 14
Screening for inhibitors of microtubule-associated motor proteins
Frank Kozielski, Salvatore DeBonis and Dimitrios Skoufias

Chapter 15 (review)
Clinical pharmacology and use of microtubule-targeting agents in
cancer therapy
Amelia B. Zelnak

Chapter 16
Studying drug-tubulin interactions by X-ray crystallography
Audrey Dorleans, Marcel Knossow and Beno? Gigant

Chapter 17
Characterizing ligand-microtubule binding by competition methods
Jos?Fernando D?z and Rub? Mart?ez Buey

Chapter 18
Methods for studying vinca alkaloid interactions with tubulin
Sharon Lobert and John J. Correia

Chapter 19
High-throughput screening of microtubule-interacting drugs
Susan L. Bane, Rudravajhala Ravindra and Anna A. Zaydman

Chapter 20 (review)
Strategies for the development of novel taxol-like agents
Susan L. Mooberry