WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

This definitive and essential source of reference has been thoroughly
updated and revised to meet the requirements of all laboratories
involved in the analysis of human semen. The book sets out the
fundamental laboratory techniques that should be employed in the
diagnosis of male infertility. The text includes descriptions of how
to construct a conventional semen profile and provides standardized
protocols for performing several optional diagnostic procedures.
Previous editions of this volume have established themselves as the
gold standard in the area of fertility investigation and treatment
and this new edition will set new standards for the new millennium.


Reviews
"This manual would be a valuable companion to both small and large
medical laboratories, as well as to andrology labs...This is a good
manual and I would personally recommend it to any laboratory that
performs semen analysis. It would also be a good reference tool in
forensic and artificial insemination laboratories." Canadian Journal
of Medical Laboratory Science

"The WHO Manual should have a prominent place on the benches of all
laboratories dealing with human semen and on the shelves of
clinicians dealing with subfertile couples... The new fourth edition
or the 'gold book,' as I am sure it will be called, will then
continue to be the benchmark for setting more rigorous standards in
the future." Human Fertility
차 례
Acknowledgements
Abbreviations

1. Introduction
2. Collection and examination of human semen
2A. Standard procedures
2B. Optional tests
2C. Research tests
3. Sperm preparation techniques
4. Quality control in the andrology laboratory
5. Sperm-cervical mucus interactions

Appendices.
IA. Reference values of semen variables
IB. Nomenclature for some semen variables
II. Safety guidelines for the andrology laboratory
III. Methods for detecting leucocytes
IV. Sperm vitality techniques
V. Papanicolau staining procedures modified for spermatozoa
VI. Shorr staining procedure for sperm morphology
VII. Rapid staining procedure for sperm morphology VIII. Immunobead
test
IX. Mixed antiglobulin reaction test (MAR test)
X. Measurement of zinc in seminal plasma
XI. Measurement of fructose in seminal plasma
XII. Measurement of neutral a-glucosidase in seminal plasma
XIII. Sample instructions and record form for semen analysis
XIV. Computer-aided sperm analysis (CASA)
XV. Protocols for the zona-free hamster test
XVI. Measurement of reactive oxygen species generated by sperm
suspensions
XVII. Induced acrosome reaction assay
XVIII. Sperm preparation techniques
XIX. Quality control (QC) procedures
XX. Sample record form for cervical mucus scoring and postcoital test
XXI. Sperm-cervical mucus interaction: the capillary tube test
XXII. Basic requirements for an andrology laboratory Bibliography
Index.