Abstract:
A collection of cutting-edge techniques for detecting and quantifying
apoptosis, understanding its biochemistry, and for identifying the genes and
proteins that regulate and carry it out. Described in step-by-step detail,
these readily reproducible methods range from flow cytometry and
immunohistochemical procedures to kinase activity assays, yeast two-hybrid
screening, and the cloning of novel genes by differential expression. The
protocols follow the successful Methods in Molecular Biology?series format,
each one offering step-by-step laboratory instructions, an introduction
outlining the principle behind the technique, lists of equipment and reagents,
and tips on troubleshooting and avoiding known pitfalls. Apoptosis Methods and
Protocols constitutes a key technical reference to the significant
methodologies used in the field, as well as offering novice and experienced
researchers alike powerful tools to illuminate the phenomenon of programmed
cell death.
Reviews:
밫he calibre of the contributors to this book, make it an authoritative tome.
As it also provides insights in to how and why specific protocols should be
applied, it will be a great asset to those studying apoptosis in laboratory-
based haematology.?뺹ritish Journal of Haematology