Cancer Cell Culture : Methods and Protocols

Preface
Contributors
1 Basic Principles of Cancer Cell Culture 3
2 Essential Techniques of Cancer Cell Culture 17
3 Characterization and Authentication of Cancer Cell Lines: An Overview 33
4 Authentication of Cancer Cell Lines by DNA Fingerprinting 43
5 Cytogenetic Characterization of Tumor Cell Lines 57
6 Isolation and Culture of Colon Cancer Cell Lines 79
7 Isolation and Culture of Melanoma Cell Lines 93
8 Isolation and Culture of Human Brain Tumor Cells 101
9 Isolation and Culture of Renal Cancer Cell Lines 111
10 Isolation and Culture of Prostate Cancer Cell Lines 121
11 Isolation and Culture of Ovarian Cancer Cell Lines 133
12 Isolation and Culture of Leukemia Cell Lines 141
13 Cell Sensitivity Assays: Clonogenic Assay 159
14 Cell Sensitivity Assays: The MTT Assay 165
15 PARP Cleavage as a Means of Assessing Apoptosis 171
16 Detection of Apoptosis by the TUNEL Assay 183
17 Apoptosis Measurement by Annexin V Staining 191
18 Cell Adhesion Assays 203
19 Radial Monolayer Cell Migration Assay 219
20 Invasion and Motility Assays 225
21 Identification of Senescence in Cancer Cells 231
22 Angiogenesis Assays 239
23 Flow Cytometric DNA Analysis of Human Cancer Cell Lines 247
24 DNA-Mediated Gene Transfer 259
25 Development of Drug-Resistant Models 267
26 Immortalization of Human Prostate Cells With the Human Papillomavirus Type
16 E6 Gene 275
27 The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell
Lines as Xenografts 287
28 Coculture of Prostate Cancer Cells 295
29 Coculture of Ovarian Cells Using Porous Tissue Culture Inserts 303
30 Cell Culture Contamination: An Overview 309
31 Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain
Reaction 319
32 Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics 327
App. 1 Formulations of Commonly Used Cell Culture Media 335
App. 2 Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks
(Combined List) 341
Index 349