Methods in Alcohol-Related Neuroscience Research-1판

Ch. 1 Quantitative Trait Locus (QTL) Mapping in Mice 1
Ch. 2 Knockout and Knockin Mice 31
App. 2.1 Preparation of Mouse Embryonic Fibroblast Feeder Cells
57
App. 2.2 ES Cell Transformation by Electroporation 59

Ch. 3 Molecular Studies by Chimeragenesis and Mutagenesis 63

Ch. 4 Combining Patch-Clamp Recording and Gene Profiling in
Single Neurons 83
App. 4.1 Protocol for Single-Cell mRNA Amplification/Reverse
Northern Analysis 99
App. 4.2 Protocol for Single-Cell RT-PCR 109
App. 4.3 Protocol for Single-Cell Multiplex RT-PCR 111

Ch. 5 Quantitative Analysis of Neurotransmitter Receptor
Expression with Antibody-Based Techniques 113
App. 5.1 Detailed Protocols for Western Immunoblotting 139

Ch. 6 Artificial Bilayer Techniques in Ion Channel Study 143

Ch. 7 Rapid Drug Superfusion and Kinetic Analysis 159

Ch. 8 Electrophysiological Assessment of Synaptic Transmission
in Brain Slices 191

Ch. 9 Cognitive Correlates of Single Neuron Activity in Task-
Performing Animals 219

Ch. 10 Multichannel Neural Ensemble Recording During Alcohol
Self-Administration 241

Ch. 11 Combined Single-Neuron Recording and Microdialysis in the
Brain of Freely Behaving Animals (Rodents and Non-Human
Primates) 259

Ch. 12 Quantitative Microdialysis for in vivo Studies of
Pharmacodynamics 287

ch. 13 Structural Magnetic Resonance Imaging (MRI) of the Human
Brain 319

Ch. 14 Biochemical, Functional and Microstructural Magnetic
Resonance Imaging (MRI) 345

Index 373