Adhesion Proteins Protocols:MMB vol.96

Production of Monoclonals Directed to Adhesive Molecules.
Monoclonal Antibodies Specific for Leucocyte Adhesion Molecules:
Selective Protocols of Immunization and Screening Assays for
Generation of Blocking, Activating, and Activation Reporter
Antibodies, C. Cabanas and F. Sanchez-Madrid. Epitope Mapping,
H.M. DeLisser. Sequencing of Antibodies, A. Jarrin and A.
Andrieux. Isolation and Cloning of Adhesive Molecules.
Purification Through Affinity Chromatography and
Microsequencing, C. Garlanda. Functional Analysis of Cell
Adhesion Molecules, D. Simmons. Application of Polymerase Chain
Reaction for the Discovery of New Adhesion Molecule Family
Members, A.S. Woodard and L.R. Languino. Construction and
Purification of Adhesion Molecule-Immunoglobulin Chimeric
Proteins, T. Moll and D. Vestweber. Morphological Localization
of Adhesive Molecules and Related Cytoskeletal Components.
Immunofluorescence of Cultured Cells, P.C. Marchisio and L.
Trusolino. Detection of Adhesion Molecules by
Immunohistochemistry on Human and Murine Tissue Sections, A.
Stopacciaro and L.P. Ruco. In situ Hybridization with RNA
Probes, G. Breier. Adhesion of Circulating Cells to the
Endothelium and to Extracellular Matrix Proteins. Purification
of Fibronectin and Vitronectin from Human Plasma, S.F. Retta, P.
Ferraris, and G. Tarone. Adhesion to Matrix Proteins, S.F.
Retta, M. Ternullo, and G. Tarone. Leucocyte-Endothelial
Monolayer Adhesion Assay (Static Conditions), J.M. Kiely, F.W.
Luscinskas, and M.A. Gimbrone. Cell-Cell Adhesive Interactions
in an In Vitro Flow Chamber, D.J. Goetz, D.M. Greif, J. Shen,
and F.W. Luscinskas. Adhesion of Tumor Cells to Endothelium
Under Static Conditions, R.G.S. Chiarivi and R.Giavazzi.
Adhesion of Tumor Cell Under Flow, A. Remuzzi and R. Giavazzi.
Platelet Adhesion to the Subendothelium Under Flow. Platelet
Adhesion to the Subendothelium Under Flow, G. de Groot, M.J.W.
Ijsseldijk, and J.J. Sixma. Cell Chemotaxis. Leucocyte
Transmigration Through Vascular Endothelium: AnIn Vitro Method,
P. Allavena and A. Del Maschio. Cell Migration into a Wounded A
rea In Vitro, M.G. Lampugnani. Endothelial Cell Permeability In
Vitro. Determination of the Endothelial Barrier Function In
Vitro, G.P. van Nieuw Amerongen and V.W.M. van Hinsbergh.
Induction of Intercellular Signals Through Binding of Adhesive
Molecules. Activation of p125 FAK Tyrosine Phosphorylation, M.
Venturino, C. Olivo, G. Tarone and P. Defilippi. Induction of
Ca2+ Flux by Adhesin Molecules in Lymphocytes, R. DeMaria, G.
Palmieri, and A. Santoni. Integrin-Mediated Stimulation of
Tyrosine Phosphorilation in Lymphoid Cells, A. Gismondi and A.
Santoni. The Identification of Signaling Molecules by the Yeast
2-Hybrid System, S. Biffo and P.C. Marchisio. Index.