An Atlas of Preimplantation Genetic Diagnosis

Foreword
Preface
Introduction
Section 1 Review of methods and experience in preimplantation
genetic diagnosis: Normal and abnormal preimplantation
development: selection of material for preimplantation genetic
diagnosis: Oocytes at different stages of meiosis I. Oocytes at
metaphase II and variations in the first polar body formation.
Oocytes following fertilization and second polar body extrusion.
Normally and abnormally cleaving embryos. Normal and abnormal
blastocyst formation. Micromanipulation and biopsy of polar
bodies and blastomeres: First polar body removal.
Intracytoplasmic sperm injection after polar body removal. First
and second polar body removal. Blastomere biopsy. Nuclear
transfer techniques for visualization of chromosomes in polar
bodies and blastomeres: Conversion of second polar body into
metaphase chromosomes. Conversion of single blastomeres into
metaphase chromosomes. Fluorescence in situ hybridization
analysis of polar bodies and blastomeres: Preparations of polar
bodies and blastomeres for fluorescence in situ hybridization
analysis. Fluorescence in situ hybridization procedure.
Fluorescent signal interpretation. PGD for chromosomal disorders
by fluorescence in situ hybridization. Single-cell DNA analysis
in polar bodies and blastomeres: Principles of PGD using first
and second polar bodies. PCR analysis in single cells. Nested
amplification. PGD for single-gene disorders. Future
perspectives.
Section 2 Preimplantation genetic diagnosis illustrated. Index.