Cancer Cytogenetics : Methods and Protocols

Cancer Cytogenetics: Methods and Protocols

SYNOPSIS
Offers hospitals and other pathology laboratories a collection of standard and
advanced techniques for improving the diagnosis and clinical management of
cancer patients. The volume's 18 contributions, written by experienced
malignancy cytogeneticists, describe in detail the key cytogenetic and CGH,
FISH, and M-FISH techniques used by modern clinical laboratories in the genetic
analysis of human malignancies, in addition to information on the
interpretation of findings, the establishment of a diagnostic service, and the
training of staff. Suitable for various levels of expertise, from those
considering setting up a new cytogenetics service to fully established
laboratories. Annotation ?003 Book News, Inc., Portland, OR


Cancer Cytogenetics: Methods and Protocols

TABLE OF CONTENTS
Preface
Contributors
1 Introduction 1
2 Cytogenetic Studies in Hematologic Malignancies: An Overview 9
3 The Myeloid Disorders: Background 23
4 Cytogenetic Techniques for Myeloid Disorders 43
5 Acute Lymphoblastic Leukemia: Background 59
6 Conventional Cytogenetic Techniques in the Diagnosis of Childhood Acute
Lymphoblastic Leukemia 73
7 Chromosome Preparations from Bone Marrow in Acute Lymphoblastic Leukemia:
Cytogenetic Techniques 83
8 Lymphoid Disorders Other than Common Acute Lymphoblastic Leukemia: Background
93
9 Other Lymphoid Malignancies: Cytogenetic Techniques 111
10 Cytogenetic and Genetic Studies in Solid Tumors: Background 125
11 Human Solid Tumors: Cytogenetic Techniques 135
12 Analysis and Interpretations of Cytogenetic Findings in Malignancies 151
13 Cytogenetic Studies Using FISH: Background 173
14 FISH Techniqes 193
15 FISH, CGH, and SKY in the Diagnosis of Childhood Acute Lymphoblastic
Leukemia 213
16 Solving Problems in Multiplex FISH 235
17 Some Difficult Choices in Cytogenetics 245
18 Introduction to the Analysis of the Human G-Banded Karyotype 259
Index 271
About the Authors 281