Embryonic Stem Cells : Methods and Protocols

1. Methods for the Isolation and Maintenance of Murine
Embryonic Stem Cells
Roach, Marsha L.; McNeish, John D.
pp. 1-16
2 The Use of a Chemically Defined Media for the Analyses of
Early Development in ES Cells and Mouse Embryos
Proetzel, Gabriele; Wiles, Michael V.
pp. 17-26
3. Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Savatier, Pierre; Lapillonne, H??e; Jirmanova, Ludmila;
Vitelli, Luigi; Samarut, Jacques
pp. 27-33
4. Murine Embryonic Stem Cells as a Model for Stress
Proteins and Apoptosis During Differentiation
Arrigo, Andr?Patrick; Mehlen, Patrick
pp. 35-44
5 Effects of Altered Gene Expression on ES Cell
Differentiation
Fan, Yong; Chaillet, J. Richard
pp. 45-53
6. Hypoxic Gene Regulation in Differentiating ES Cells
Adelman, David M.; Simon, M. Celeste
pp. 55-62
7. Regulation of Gap Junction Protein (Connexin) Genes and
Function in Differentiating ES Cells
Oyamada, Masahito; Oyamada, Yumiko; Kaneko, Tomoyuki; Takamatsu,
Tetsuro
pp. 63-69
8. Embryonic Stem Cell Differentiation as a Model to Study
Hematopoietic and Endothelial Cell Development
Fraser, Stuart T.; Ogawa, Minetaro; Nishikawa, Satomi;
Nishikawa, Shin-Ichi
pp. 71-81
9. Analysis of Bcr-Abl Function Using an In Vitro Embryonic
Stem Cell Differentiation System
Era, Takumi; Wong, Stephane; Witte, Owen N.
pp. 83-95
10. Embryonic Stem Cells as a Model for Studying Osteoclast
Lineage Development
Yamane, Toshiyuki; Kunisada, Takahiro; Hayashi, Shin-Ichi
pp. 97-106

11. Differentiation of Embryonic Stem Cells as a Model to
Study Gene Function During the Development of Adipose Cells
Dani, Christian
pp. 107-116

12. Embryonic Stem Cell Differentiation and the Vascular
Lineage
Bautch, Victoria L.
pp. 117-125
13. Embryonic Stem Cells as a Model to Study Cardiac,
Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
Wobus, Anna M.; Guan, Kaomei; Yang, Huang-Tian; Boheler, Kenneth
R.
pp. 127-156
14. Cardiomyocyte Enrichment in Differentiating ES Cell
Cultures: Strategies and Applications
Pasumarthi, Kishore B.S.; Field, Loren J.
pp. 157-168
15. Embryonic Stem Cells as a Model for the Physiological
Analysis of the Cardiovascular System
Hescheler, J?rgen; Wartenberg, Maria; Fleischmann, Bernd K.;
Banach, Kathrin; Acker, Helmut; Sauer, Heinrich
pp. 169-187
16. Isolation of Lineage-Restricted Neural Precursors from
Cultured ES Cells
Mujtaba, Tahmina; Rao, Mahendra S.
pp. 189-204
17. Lineage Selection for Generation and Amplification of
Neural Precursor Cells
Li, Meng
pp. 205-215
18. Selective Neural Induction from ES Cells by Stromal Cell-
Derived Inducing Activity and Its Potential Therapeutic
Application in Parkinson's Disease
Kawasaki, Hiroshi; Mizuseki, Kenji; Sasai, Yoshiki
pp. 217-227
19. Epidermal Lineage
Troy, Tammy-Claire; Turksen, Kursad
pp. 229-253
20. ES Cell Differentiation Into the Hair Follicle Lineage
In Vitro
Troy, Tammy-Claire; Turksen, Kursad
pp. 255-260
21. Embryonic Stem Cells as a Model for Studying Melanocyte
Development
Yamane, Toshiyuki; Hayashi, Shin-Ichi; Kunisada, Takahiro
pp. 261-268
22. Using Progenitor Cells and Gene Chips to Define Genetic
Pathways
Potter, S. Steven; Valerius, M. Todd; Brunskill, Eric W.
pp. 269-284
23. ES Cell-Mediated Conditional Transgenesis
Gertsenstein, Marina; Lobe, Corrinne; Nagy, Andras
pp. 285-307
24. Switching on Lineage Tracers Using Site-Specific
Recombination
Dymecki, Susan M.; Rodriguez, Carolyn I.; Awatramani, Rajeshwar
B.
pp. 309-334
25. From ES Cells to Mice: The Gene Trap Approach
Cecconi, Francesco; Gruss, Peter
pp. 335-346
26. Functional Genomics by Gene-Trapping in Embryonic Stem
Cells
Floss, Thomas; Wurst, Wolfgang
pp. 347-379
27. Phage-Displayed Antibodies to Detect Cell Markers
Lu, Jun; Sloan, Steven R.
pp. 381-392
28. Gene Transfer Using Targeted Fil