Cytokine Molecular Biology:Practical Approach

List of protocols
Abbreviations
1 Cloning and expression of cytokine genes 1
2 Detection and population analysis of IL-1 and TNF gene
polymorphisms 21
3 Northern analysis, RT-PCR, RNase protection, cDNA array
systems, and In Situ hybridization to detect cytokine messenger
RNA 47
4 Purification, sequencing, and synthesis of cytokines and
chemokines 73
5 Receptor binding studies 89
6 Cytokine signalling 105
7 Cytokine receptor signalling pathways: PC-specific
phospholipase C and sphingomyelinases 133
8 Conditional and fixed cytokine gene modifications in
transgenic and mutant mice 155
A1: List of suppliers 189
Index 199